grna library addgene Search Results


lentiviral grna library  (Addgene inc)
93
Addgene inc lentiviral grna library
( A ) Schematic of the retroviral V(D)J recombination substrate pMG-INV. The open and filled triangles represent the RSSs. The retroviral LTRs, CEs, and SEs upon RAG cleavage and CJs and SJs after NHEJ-mediated repair are indicated. The anti-sense GFP cDNA is inverted to the sense orientation upon completion of the recombination and its expression driven by the retroviral LTR (green). ( B ) Schematic diagram of a genome-wide CRISPR-Cas9 screen in WT abl pre-B cells for identifying genes important for V(D)J recombination upon DNA-PKcs inhibition with NU7441. ( C ) Fold enrichments of selected gRNAs from screens conducted in DMSO and NU7441-treated WT abl pre-B cells (both also treated with imatinib). The fold enrichment is calculated as the ratio of the normalized read counts of a <t>gRNA</t> from GFP − cells over that from GFP + cells. Asterisk denotes (*) five gRNAs to each gene. ( D ) Flow cytometric analysis for GFP expression in WT and three independently isolated Setx −/− abl pre-B cell lines with pMG-INV and treated with imatinib (imat.) in the presence or absence of the DNA-PKcs kinase inhibitor NU7441 for the indicated times. The percentages of GFP + cells are indicated in the top left corners of the histograms.
Lentiviral Grna Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lentiviral grna library/product/Addgene inc
Average 93 stars, based on 1 article reviews
lentiviral grna library - by Bioz Stars, 2026-04
93/100 stars
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human genome wide library  (Addgene inc)
94
Addgene inc human genome wide library
( A ) Schematic of the retroviral V(D)J recombination substrate pMG-INV. The open and filled triangles represent the RSSs. The retroviral LTRs, CEs, and SEs upon RAG cleavage and CJs and SJs after NHEJ-mediated repair are indicated. The anti-sense GFP cDNA is inverted to the sense orientation upon completion of the recombination and its expression driven by the retroviral LTR (green). ( B ) Schematic diagram of a genome-wide CRISPR-Cas9 screen in WT abl pre-B cells for identifying genes important for V(D)J recombination upon DNA-PKcs inhibition with NU7441. ( C ) Fold enrichments of selected gRNAs from screens conducted in DMSO and NU7441-treated WT abl pre-B cells (both also treated with imatinib). The fold enrichment is calculated as the ratio of the normalized read counts of a <t>gRNA</t> from GFP − cells over that from GFP + cells. Asterisk denotes (*) five gRNAs to each gene. ( D ) Flow cytometric analysis for GFP expression in WT and three independently isolated Setx −/− abl pre-B cell lines with pMG-INV and treated with imatinib (imat.) in the presence or absence of the DNA-PKcs kinase inhibitor NU7441 for the indicated times. The percentages of GFP + cells are indicated in the top left corners of the histograms.
Human Genome Wide Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human genome wide library/product/Addgene inc
Average 94 stars, based on 1 article reviews
human genome wide library - by Bioz Stars, 2026-04
94/100 stars
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pre amplified  (Addgene inc)
92
Addgene inc pre amplified
( A ) Schematic of the retroviral V(D)J recombination substrate pMG-INV. The open and filled triangles represent the RSSs. The retroviral LTRs, CEs, and SEs upon RAG cleavage and CJs and SJs after NHEJ-mediated repair are indicated. The anti-sense GFP cDNA is inverted to the sense orientation upon completion of the recombination and its expression driven by the retroviral LTR (green). ( B ) Schematic diagram of a genome-wide CRISPR-Cas9 screen in WT abl pre-B cells for identifying genes important for V(D)J recombination upon DNA-PKcs inhibition with NU7441. ( C ) Fold enrichments of selected gRNAs from screens conducted in DMSO and NU7441-treated WT abl pre-B cells (both also treated with imatinib). The fold enrichment is calculated as the ratio of the normalized read counts of a <t>gRNA</t> from GFP − cells over that from GFP + cells. Asterisk denotes (*) five gRNAs to each gene. ( D ) Flow cytometric analysis for GFP expression in WT and three independently isolated Setx −/− abl pre-B cell lines with pMG-INV and treated with imatinib (imat.) in the presence or absence of the DNA-PKcs kinase inhibitor NU7441 for the indicated times. The percentages of GFP + cells are indicated in the top left corners of the histograms.
Pre Amplified, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pre amplified/product/Addgene inc
Average 92 stars, based on 1 article reviews
pre amplified - by Bioz Stars, 2026-04
92/100 stars
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library backbone plasmid  (Addgene inc)
86
Addgene inc library backbone plasmid
( A ) Schematic of the retroviral V(D)J recombination substrate pMG-INV. The open and filled triangles represent the RSSs. The retroviral LTRs, CEs, and SEs upon RAG cleavage and CJs and SJs after NHEJ-mediated repair are indicated. The anti-sense GFP cDNA is inverted to the sense orientation upon completion of the recombination and its expression driven by the retroviral LTR (green). ( B ) Schematic diagram of a genome-wide CRISPR-Cas9 screen in WT abl pre-B cells for identifying genes important for V(D)J recombination upon DNA-PKcs inhibition with NU7441. ( C ) Fold enrichments of selected gRNAs from screens conducted in DMSO and NU7441-treated WT abl pre-B cells (both also treated with imatinib). The fold enrichment is calculated as the ratio of the normalized read counts of a <t>gRNA</t> from GFP − cells over that from GFP + cells. Asterisk denotes (*) five gRNAs to each gene. ( D ) Flow cytometric analysis for GFP expression in WT and three independently isolated Setx −/− abl pre-B cell lines with pMG-INV and treated with imatinib (imat.) in the presence or absence of the DNA-PKcs kinase inhibitor NU7441 for the indicated times. The percentages of GFP + cells are indicated in the top left corners of the histograms.
Library Backbone Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/library backbone plasmid/product/Addgene inc
Average 86 stars, based on 1 article reviews
library backbone plasmid - by Bioz Stars, 2026-04
86/100 stars
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Image Search Results


( A ) Schematic of the retroviral V(D)J recombination substrate pMG-INV. The open and filled triangles represent the RSSs. The retroviral LTRs, CEs, and SEs upon RAG cleavage and CJs and SJs after NHEJ-mediated repair are indicated. The anti-sense GFP cDNA is inverted to the sense orientation upon completion of the recombination and its expression driven by the retroviral LTR (green). ( B ) Schematic diagram of a genome-wide CRISPR-Cas9 screen in WT abl pre-B cells for identifying genes important for V(D)J recombination upon DNA-PKcs inhibition with NU7441. ( C ) Fold enrichments of selected gRNAs from screens conducted in DMSO and NU7441-treated WT abl pre-B cells (both also treated with imatinib). The fold enrichment is calculated as the ratio of the normalized read counts of a gRNA from GFP − cells over that from GFP + cells. Asterisk denotes (*) five gRNAs to each gene. ( D ) Flow cytometric analysis for GFP expression in WT and three independently isolated Setx −/− abl pre-B cell lines with pMG-INV and treated with imatinib (imat.) in the presence or absence of the DNA-PKcs kinase inhibitor NU7441 for the indicated times. The percentages of GFP + cells are indicated in the top left corners of the histograms.

Journal: Science Advances

Article Title: Senataxin and DNA-PKcs redundantly promote non-homologous end joining repair of DNA double strand breaks during V(D)J recombination

doi: 10.1126/sciadv.ads5272

Figure Lengend Snippet: ( A ) Schematic of the retroviral V(D)J recombination substrate pMG-INV. The open and filled triangles represent the RSSs. The retroviral LTRs, CEs, and SEs upon RAG cleavage and CJs and SJs after NHEJ-mediated repair are indicated. The anti-sense GFP cDNA is inverted to the sense orientation upon completion of the recombination and its expression driven by the retroviral LTR (green). ( B ) Schematic diagram of a genome-wide CRISPR-Cas9 screen in WT abl pre-B cells for identifying genes important for V(D)J recombination upon DNA-PKcs inhibition with NU7441. ( C ) Fold enrichments of selected gRNAs from screens conducted in DMSO and NU7441-treated WT abl pre-B cells (both also treated with imatinib). The fold enrichment is calculated as the ratio of the normalized read counts of a gRNA from GFP − cells over that from GFP + cells. Asterisk denotes (*) five gRNAs to each gene. ( D ) Flow cytometric analysis for GFP expression in WT and three independently isolated Setx −/− abl pre-B cell lines with pMG-INV and treated with imatinib (imat.) in the presence or absence of the DNA-PKcs kinase inhibitor NU7441 for the indicated times. The percentages of GFP + cells are indicated in the top left corners of the histograms.

Article Snippet: For each screen, ~180 × 10 6 cells were transduced with a lentiviral gRNA library containing 90,230 gRNAs targeting 18,424 mouse genes (Addgene, #67988) at a 40 to 50% transduction efficiency, as determined by the percentage of blue fluorescent protein (BFP)–positive cells after transduction.

Techniques: Retroviral, Expressing, Genome Wide, CRISPR, Inhibition, Isolation